CMV-associated encephalitis and antineuronal autoantibodies - a case report
- Xinling Xu†1,
- Peter Bergman†2, 7Email author,
- Thomas Willows3,
- Charlotte Tammik1,
- Marie Sund4,
- Tomas Hökfelt5,
- Cecilia Söderberg-Naucler†1 and
- Stefania Varani6
© Xu et al.; licensee BioMed Central Ltd. 2012
Received: 11 May 2012
Accepted: 28 August 2012
Published: 4 September 2012
Human cytomegalovirus (CMV) is an ubiquitous pathogen capable of modulating the host immune system. Immune dysfunction is common during CMV infection and includes autoimmune phenomena. Here we focus on a case of primary CMV infection associated with encephalopathy in a patient with a rudimentary spleen. We discuss diagnostic challenges and immunological aspects as well as the hypothesis that CMV may break tolerance and induce potentially encephalitogenic autoantibodies.
A 33-year-old woman was admitted with features of encephalitis, rapidly progressing into a catatonic state. The patient tested negative for presence of herpes simplex virus DNA in cerebrospinal fluid (CSF), and had elevated liver enzymes and hepatomegaly at computed tomography scan (CT) examination. CT scan and magnetic resonance imaging (MRI) showed only a rudimentary spleen. Initially, serum was negative for anti-CMV IgM, but borderline for anti-CMV IgG by enzyme-linked immunosorbent assay. However, a more sensitive assay resulted in a positive specific IgM Western blot profile and low IgG avidity, suggesting primary CMV infection. Further, CMV DNA was retrospectively detected in a CSF sample collected at admission. We also detected antineuronal autoantibodies, which stained GAD-positive neurons in the hippocampus. The patient was treated by a combination of prednisone, intravenous immunoglobulins (IVIg) and antivirals, which resulted in a dramatic amelioration of the patient’s neurological status. One year after admission the patient exhibited a nearly complete recovery with mild deficits in attention and memory.
A possible reason for the critical course of CMV infection could be the lack of a functional spleen in this patient, a condition previously associated with severe CMV infection. Prompt treatment with antiviral drugs, steroids and IVIg was most likely important for the positive outcome in this case and should be considered for similar cases of severe primary CMV infection associated with immunopathological phenomena.
KeywordsHuman cytomegalovirus Encephalitis Antineuronal autoantibodies
Human cytomegalovirus (CMV) is an ubiquitous pathogen capable of modulating the host immune system. Immune dysfunction is common during CMV infection and include autoimmune phenomena [1, 2] and Guillain-Barré syndrome , the latter suggesting that CMV may contribute to neuropathological processes. In patients with autoimmune diseases, the virus can replicate at sites of inflammation , highlighting CMV’s potential role in inducing or maintaining immunopathology. Here we focus on a case of primary CMV infection associated with encephalopathy in a patient with a rudimentary spleen. We discuss diagnostic challenges and immunological aspects as well as the hypothesis that CMV may break tolerance and induce potentially encephalitogenic autoantibodies. Finally, the clinical utility of antivirals combined with intravenous immunoglobulins (IVIg) and prednisone in the treatment of severe CMV infections is highlighted.
On Day 9 after admission, a short loss of consciousness was reported, and on Day 10 the patient was found unconscious, with tonic extensions/spasms of the extremities, involuntary loss of urine and tongue bites. Despite administration of Diazepam and Fosphenytoin, the patient remained unconscious and was transferred to the Intensive Care Unit (ICU) (Figure 1B). She thereafter entered a catatonic condition. Continuous electroencephalography (EEG) monitoring showed frontal bilateral sparse hints of sharp waves/epileptic activity but no certain seizure activity. After this episode, the patient had several seizures in form of tonic extensions/spasms of the extremities with autonomic influence and she was treated at the ICU at 4 more occasions. Repeated EEG examinations did not show any epileptic or seizure activity even when the patient experienced tonic extensions/spasms.
At Week 3, the patient presented myoclonic jerks in all four extremities with irregular pattern, which were triggered by auditory and sensory stimuli. Occasionally, the patient also exhibited ophistotonus. EEG examinations was performed while the patient exhibited these jerks, but no signs of epileptic changes or seizure activity were found, suggesting cortical myoclonus and brainstem evoked decerebration seizures.
Computed tomography (CT) and magnetic resonance imaging (MRI) head scans did not show any signs of cerebrovascular insult, or malignancy. Nevertheless, accumulation of contrast signal was detected by MRI in pons, brainstem, thalamus and in the cerebellar leptomeninges (Figure 1C), which was judged as a non-specific sign of inflammation.
The patient had elevated liver enzymes (Figure 1A and data not shown) and hepatomegaly at CT examination, which led to the suspicion of autoimmune liver disease. In support of this, the patient exhibited hypergammaglobulinemia and anti-nuclear, anti-smooth muscle cell and anti-SS-A52 autoantibodies. Several other autoantibodies were also detected, including anti-MitoM2, M2-3E, gp120, Sp100, PML and anti-mitochondrial autoantibodies indicating primary biliary cirrhosis as well as anti-Ro52, anti-Ro60, SS-B and anti-dsDNA antibodies as in Sjögren’s syndrome or systemic lupus erythematosus. However, a biopsy of the lip salivary gland was negative for histological hallmarks of Sjögren’s syndrome and no other systemic autoimmune disease could be confirmed.
CT scan and MRI showed only a rudimentary spleen. Notably, the patient’s former medical history of ITP did not involve splenectomy.
At Week 23 after disease onset the total T-cell count was normal (5x109 cells/L), but low levels of CD4+ T cells and increased levels of CD8+ T cells were observed (38% and 87% of total T cells, respectively). The B-cell count was lower than normal and T- and B-cell proliferation upon mitogen stimulation was impaired (data not shown).
At admission, serum and CSF samples tested negative for varicella–zoster virus, borrelia, Treponema pallidum, and toxoplasma and revealed past contact for Epstein-Barr virus, adenovirus, and measles. Of note, a serum sample tested negative for anti-CMV IgM, but borderline for anti-CMV IgG by enzyme-linked immunosorbent assay. Because of the unclear result for specific IgG, a blood sample was analysed at Week 5 and tested positive for CMV DNA, indicating active CMV replication (Figure 1B). A CSF sample collected at admission was retrospectively analysed and CMV DNA was detected by PCR. Two serum samples, obtained from the patient at admission and ten days later, retrospectively showed a positive specific IgM profile by immunoblot analysis (method described in ) and low IgG avidity, suggestive of primary CMV infection.
Empirical treatment with intravenous high-dose cortisone was initiated during Week 3 (Figure 1B), followed by oral prednisone and intravenous immunoglobulins (IVIg) at Week 4. When the diagnosis of active CMV infection was confirmed, intravenous ganciclovir treatment was initiated in combination with prednisone; administration of antivirals and steroids was continued daily in a course of 5 months, while IVIg were administered in an intermittent way in nine doses (Figure 1B and data not shown). This therapy resulted in a dramatic amelioration of the patient’s neurological status. Radiological examinations of the brain showed regression of pathological signs (Figure 1). Liver enzymes normalized (Figure 1A). CMV DNA load decreased and then tested negative (Figure 1B). After four months, the patient was discharged to a rehabilitation ward. She remained on antivirals for a total of five months while prednisone was diminished on a scalar basis until complete discontinuation after 11 months. One year after admission the patient exhibited a nearly complete cognitive recovery with mild deficits in attention and memory. At neurological examination the only sequelae were oscillating eye movement at abduction, minor sporadic myoclonus and slightly impaired balance. The patient is now living by herself and attending college.
At Week 12 low levels of BDCA-1+ (CD1c) and BDCA-3+ (CD141) myeloid dendritic cells (DCs) and BDCA-2+ (CD303) plasmacytoid DC were detected and remained impaired up to Week 24 (Figure 2C and D).
Since the patient presented with clinical signs of limbic encephalitis (cognitive impairment, seizures and pathologic MRI-scan), the presence of autoantibodies against antigens known to be involved in triggering this pathological condition was investigated . Serum and CSF samples were negative for anti-Hu, anti-Ma, anti-voltage gated potassium channel, anti-collapsin response mediator protein-5 as well as for autoantibodies against the N-methyl-D-aspartate (NMDA) receptor. Further, we screened for the presence of neuronal autoantibodies, by applying patient serum to sections of murine brains as described . A strong staining in neurons of cortex and hippocampus but not in glial cells was observed (Figure 2E and F). To assess the specificity of this finding, sera from 3 healthy CMV IgG-negative and 6 CMV IgG-positive controls were investigated; very weak or absent staining was observed (Figure 2G and data not shown). Interestingly, many – but not all – neurons in hippocampus that were stained by the patient serum were also immunoreactive for glutamic acid decarboxylase (GAD) (Figure 2H, inset).
Here, we describe a case of primary CMV infection associated with encephalitis and autoimmune phenomena in a young woman. This case was unusual for several reasons: i) CMV infections involving the central nervous system are uncommon in previously healthy individuals ; ii) an impaired CMV-specific T-cell response was observed for several months after infection; iii) the patient developed antineuronal autoantibodies directed against neurons in the hippocampus.
First, the patient exhibited a rudimentary spleen, which could have contributed to the lack of development of a proper anti-CMV immune response leading to severe infection. In fact, the spleen is crucial for development of IgM memory B-cells, which are important for immunity against viruses, including CMV  and splenectomised patients have been described to suffer from more severe CMV infections than eusplenic patients .
The poor IgM development against CMV in patients with a dysfunctional or absent spleen may complicate diagnostic procedures . In our case, IgM against CMV tested negative during the active phase of infection by employing a commercial immunoenzymatic assay, which delayed the time to diagnosis. The diagnosis was eventually established through detection of circulating CMV DNA. Further, by the use of a more sensitive IgM Western blot method, IgM antibodies could be detected, which confirmed the diagnosis of active CMV infection.
The immunological status of the patient at disease onset showed low B-cell count as well as deficient lymphocyte proliferative response to mitogens. As a further sign of impaired immunity, circulating DC count was low; this is a common finding during primary symptomatic CMV infection in adults, as we recently reported . Cytotoxic T lymphocytes (CTL) appear to play an important role in the control of CMV infection, 70-90% of all CTL being specific for the viral antigen pp65 . Importantly, CD4+ and CD8+ T-cell responses to pp65 remained impaired up to Week 62 in our patient, while high plasma levels of IFN-γ suggest activation of innate mechanisms to compensate for the lack of CMV-specific responses. We hypothesize that uncontrolled viral replication in a subject with previously hidden immune-defects may have caused the virus to reach the central nervous system, thus triggering viral encephalitis. However, we cannot exclude that the virus per se may have contributed to impeding the host response. In fact, it is known that CMV inhibits antigen presenting cell function leading to impaired T cell responses (as reviewed in ) and alters mitogen-induced lymphocyte proliferation [16, 17].
A third unusual feature of this case is that we detected autoantibodies with strong specificity to neurons; primary CMV infection may have contributed to the break of self-tolerance and to a broad polyclonal activation of B-cell clones with the final result of several autoantibodies, including those binding to neurons. Many neuronal antibodies stained cells that were GAD-positive, suggesting that the GABAergic system might be involved in the development of the observed immunopathology as anti-GAD autoantibodies have been linked to cerebellar ataxia and seizures . However, since not all serum IgG-positive cells expressed GAD, other autoantigen(s) may be involved in the actual case.
A key observation that links active CMV infection and viral-induced immunopathology to the overall clinical presentation is the fact that the onset of a combined treatment including antivirals, immunomodulatory IVIg and prednisone coincided with a rapid improvement of the patient’s clinical status (Figure 1B). Therapeutical effect of single drugs could not be discerned, as ganciclovir and immunomodulatory drugs were administered simultaneously. It is known that antivirals are effective in the management of direct effects caused by CMV replication in immunocompromised patients , while optimal therapy for treating virus-induced immunopathology remains obscure; it is possible that IVIg and prednisone contributed to the positive outcome in this case.
The patient described here had a primary CMV infection, which was associated with clinical and radiological signs of encephalitis. A possible reason for the critical course of CMV infection could be the lack of a functional spleen in this patient, a condition previously associated with severe CMV infection. Supporting this possibility, we observed poorly responding T cells to CMV antigens which lasted several months after the infection’s onset. CMV encephalitis in immunocompetent individuals has been associated with mild signs of inflammation of the CNS, including absent to moderate pleocytosis [19–21]. Our patient exhibited normal cell count and the presence of CMV DNA in the CSF at disease’s onset. Our main hypothesis is that primary CMV infection was the first insult and had a fulminant course because of the patient’s immune status (asplenic). Tolerance was broken and autoimmunity ensued. Infection of the CNS and general inflammation may have injured the blood brain barrier, thus allowing pathogenetic serum antibodies to enter the brain and cause neuronal damage. In support to our hypothesis, the need of an inflammatory trigger to allow serum antibodies to bind neurons in the brain has been demonstrated in a murine model of lupus . Because autoantibodies binding to GAD-positive neurons were detected in this patient, we propose that the emerging concept of autoantibody-mediated encephalitis should be included in the differential diagnostic algorithm for patients presenting with signs of viral encephalitis as the two conditions may overlap. Prompt treatment with antiviral drugs, steroids and IVIg was most likely important for the positive outcome in this case and should be considered for similar cases of severe primary CMV infection associated with immunopathological phenomena.
Written informed consent was obtained from the patient for publication of this Case report and any accompanying images. A copy of the written consent is available for review by the Series Editor of this journal.
XX: MD, PhD-student, Currently doing her internship; PB: MD, PhD, PostDoc, Residency in Clinical Microbiology; TW: MD, Specialist in Neurology; CT: MSc, LabManager, Immunology; MS: MD, Residency in Radiology; TH: MD, PhD, Professor emeritus in Neuroanatomy; CS-N: MD, PhD, Professor in Microbial Pathogenesis; SV: MD, PhD, Assistant Professor, Specialist in Microbiology and Virology.
Blood Dendritic Cell Antigen
Glutamic Acid Decarboxylase
Herpes Simplex Virus
Idiopathic Thrombocytopenic purpura
Magnetic Resonance Imaging.
This work was supported by the Swedish Research Council [to TH and CS-N], the Swedish Society of Medicine, the Tobias Foundation, the Swedish Children Cancer Research Foundation, the Heart and Lung Foundation, and the Stockholm County Council, Sweden [to CS-N] and the RFO2009 from the University of Bologna, Italy [to SV]. PB holds a postdoctoral position financed by Karolinska Institutet and the Stockholm County Council and is supported by the DA Hagelen, Åke Wiberg and Magnus Bergwall foundations, Karolinska Institutet, and the Swedish Association for Medical Microbiology.
- Hebart H, Einsele H, Klein R, Fischer I, Buhler S, Dietz K, Jahn G, Berg PA, Kanz L, Muller CA: CMV infection after allogeneic bone marrow transplantation is associated with the occurrence of various autoantibodies and monoclonal gammopathies. Br J Haematol. 1996, 95 (1): 138-144. 10.1046/j.1365-2141.1996.7592380.x.View ArticlePubMedGoogle Scholar
- Soderberg C, Sumitran-Karuppan S, Ljungman P, Moller E: CD13-specific autoimmunity in cytomegalovirus-infected immunocompromised patients. Transplantation. 1996, 61 (4): 594-600. 10.1097/00007890-199602270-00014.View ArticlePubMedGoogle Scholar
- Orlikowski D, Porcher R, Sivadon-Tardy V, Quincampoix JC, Raphael JC, Durand MC, Sharshar T, Roussi J, Caudie C, Annane D, et al: Guillain-Barre syndrome following primary cytomegalovirus infection: a prospective cohort study. Clinical infectious diseases : an official publication of the Infectious Diseases Society of America. 2011, 52 (7): 837-844. 10.1093/cid/cir074.View ArticleGoogle Scholar
- Varani S, Landini MP: Cytomegalovirus-induced immunopathology and its clinical consequences. Herpesviridae. 2011, 2 (1): 6-10.1186/2042-4280-2-6.View ArticlePubMedPubMed CentralGoogle Scholar
- Weidmann M, Meyer-Konig U, Hufert FT: Rapid detection of herpes simplex virus and varicella-zoster virus infections by real-time PCR. J Clin Microbiol. 2003, 41 (4): 1565-1568. 10.1128/JCM.41.4.1565-1568.2003.View ArticlePubMedPubMed CentralGoogle Scholar
- Lazzarotto T, Maine GT, Dal Monte P, Ripalti A, Landini MP: A novel Western blot test containing both viral and recombinant proteins for anticytomegalovirus immunoglobulin M detection. J Clin Microbiol. 1997, 35 (2): 393-397.PubMedPubMed CentralGoogle Scholar
- Fasth AE, Dastmalchi M, Rahbar A, Salomonsson S, Pandya JM, Lindroos E, Nennesmo I, Malmberg KJ, Soderberg-Naucler C, Trollmo C, et al: T cell infiltrates in the muscles of patients with dermatomyositis and polymyositis are dominated by CD28null T cells. J Immunol. 2009, 183 (7): 4792-4799. 10.4049/jimmunol.0803688.View ArticlePubMedGoogle Scholar
- Vincent A, Bien CG, Irani SR, Waters P: Autoantibodies associated with diseases of the CNS: new developments and future challenges. Lancet Neurol. 2011, 10 (8): 759-772. 10.1016/S1474-4422(11)70096-5.View ArticlePubMedGoogle Scholar
- Fetissov SO, Hallman J, Oreland L, Af Klinteberg B, Grenback E, Hulting AL, Hokfelt T: Autoantibodies against alpha -MSH, ACTH, and LHRH in anorexia and bulimia nervosa patients. Proc Natl Acad Sci USA. 2002, 99 (26): 17155-17160. 10.1073/pnas.222658699.View ArticlePubMedPubMed CentralGoogle Scholar
- Maschke M, Kastrup O, Diener HC: CNS manifestations of cytomegalovirus infections: diagnosis and treatment. CNS Drugs. 2002, 16 (5): 303-315. 10.2165/00023210-200216050-00003.View ArticlePubMedGoogle Scholar
- Han XY, Hellerstedt BA, Koller CA: Postsplenectomy cytomegalovirus mononucleosis is a distinct clinicopathologic syndrome. Am J Med Sci. 2010, 339 (4): 395-399.View ArticlePubMedGoogle Scholar
- Assy N, Gefen H, Schlesinger S, Karim W: Reactivation versus primary CMV infection after splenectomy in immunocompetent patients. Dig Dis Sci. 2007, 52 (12): 3477-3479. 10.1007/s10620-006-9712-1.View ArticlePubMedGoogle Scholar
- Varani S, Rossini G, Mastroianni A, Tammik C, Frascaroli G, Landini MP, Castellani G, Soderberg-Naucler C: High TNF-alpha and IL-8 levels predict low blood dendritic cell counts in primary cytomegalovirus infection. Journal of clinical virology: the official publication of the Pan American Society for Clinical Virology. 2012, 53 (4): 360-363. 10.1016/j.jcv.2011.12.028.View ArticleGoogle Scholar
- Wills MR, Carmichael AJ, Mynard K, Jin X, Weekes MP, Plachter B, Sissons JG: The human cytotoxic T-lymphocyte (CTL) response to cytomegalovirus is dominated by structural protein pp 65: frequency, specificity, and T-cell receptor usage of pp 65-specific CTL. J Virol. 1996, 70 (11): 7569-7579.PubMedPubMed CentralGoogle Scholar
- Varani S, Frascaroli G, Landini MP, Soderberg-Naucler C: Human cytomegalovirus targets different subsets of antigen-presenting cells with pathological consequences for host immunity: implications for immunosuppression, chronic inflammation and autoimmunity. Rev Med Virol. 2009, 19 (3): 131-145. 10.1002/rmv.609.View ArticlePubMedGoogle Scholar
- Rinaldo CR, Carney WP, Richter BS, Black PH, Hirsch MS: Mechanisms of immunosuppression in cytomegaloviral mononucleosis. J Infect Dis. 1980, 14 (4): 488-495.View ArticleGoogle Scholar
- Rinaldo CR, Rinaldo RL: Alteration of immunoregulatory mechanisms during cytomegalovirus mononucleosis: effect of in vitro culture on lymphocyte blastogenesis to viral antigens. Clin Immunol Immunopathol. 1983, 28 (1): 46-55. 10.1016/0090-1229(83)90187-3.View ArticlePubMedGoogle Scholar
- Giometto B, Nicolao P, Macucci M, Tavolato B, Foxon R, Bottazzo GF: Temporal-lobe epilepsy associated with glutamic-acid-decarboxylase autoantibodies. Lancet. 1998, 352 (9126): 457-View ArticlePubMedGoogle Scholar
- Phillips CA, Fanning WL, Gump DW, Phillips CF: Cytomegalovirus encephalitis in immunologically normal adults. Successful treatment with vidarabine. JAMA. 1977, 238 (21): 2299-2300.PubMedGoogle Scholar
- Prosch S, Schielke E, Reip A, Meisel H, Volk HD, Einhaupl KM, Kruger DH: Human cytomegalovirus (HCMV) encephalitis in an immunocompetent young person and diagnostic reliability of HCMV DNA PCR using cerebrospinal fluid of nonimmunosuppressed patients. J Clin Microbiol. 1998, 36 (12): 3636-3640.PubMedPubMed CentralGoogle Scholar
- Studahl M, Ricksten A, Sandberg T, Bergstrom T, Elowson S: Cytomegalovirus encephalitis in four immunocompetent patients. Lancet. 1992, 340 (8826): 1045-1046.View ArticlePubMedGoogle Scholar
- Kowal C, DeGiorgio LA, Nakaoka T, Hetherington H, Huerta PT, Diamond B, Volpe BT: Cognition and immunity; antibody impairs memory. Immunity. 2004, 21 (2): 179-188. 10.1016/j.immuni.2004.07.011.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2377/12/87/prepub