Cortical laminar necrosis in dengue encephalitis—a case report
© The Author(s). 2017
Received: 4 January 2017
Accepted: 1 April 2017
Published: 20 April 2017
Dengue encephalitis is a rare neurological manifestation of dengue fever. Its clinical presentation is similar to other viral encephalitides and encephalopathy. No single specific finding on magnetic resonance imaging of dengue encephalitis has yet been documented. They are highly variable and atypical.
A 15-year boy presented with fever, the headache and altered sensorium of 12-day duration. On neurological examination, his Glasgow Coma Scale score was 10 (E3M4V3). There was no focal neurological deficit. Laboratory evaluation revealed leukopenia and marked thrombocytopenia. Dengue virus IgM antibody was positive both in serum and cerebrospinal fluid. Magnetic resonance imaging of the brain revealed signal changes in bilateral parietooccipital and left frontal regions (left hemisphere more involved than the right hemisphere). There was gyriform enhancement bilateral parietooccipital regions consistent with cortical laminar necrosis. Bilaterally diffuse subcortical white matter was also involved and subtle T2 hyperintensity involving both basal ganglia was noted. Gradient echo sequence revealed presence of hemorrhage in the subcortical white matter. Patient was treated conservatively and received platelet transfusion. Patient became fully conscious after 7 days.
In a patient with highly suggestive dengue e\ephalitis, we describe an unusual magnetic resonance imaging finding. This report is possibly the first instance of cortical laminar necrosis in such a setting.
KeywordsCortical laminar necrosis Encephalitis Encephalopathy Magnetic resonance imaging Dengue virus Case report
Currently, dengue infection is endemic in more than 100 countries. The incidence of dengue has increased dramatically in recent decades. World Health Organization, currently, estimates that 390 million dengue infections occur per year, of which 96 million manifest clinically; about 2.5% of those affected dies. Dengue virus is a single-stranded RNA virus belonging to the Flaviviridae family. There are four serotypes of dengue virus (DENV-1 to DENV-4), of which serotypes DENV-2 and DENV-3 have been implicated most frequently in dengue fever. The involvement of the central nervous system has been described with all 3 classical types of dengue, dengue fever, dengue hemorrhagic fever, and dengue shock syndrome .
Neuroimaging spectrum of dengue-associated encephalitis/encephalopathy
Diffuse brain involvement
Impaired liver and renal dysfunction Ischemic-hypoxemic injury
Usually normal; diffuse cortical and periventricular, hyperintensities
Direct viral invasion of brain
Acute hemorrhagic encephalitis
GRE sequence shows areas of blooming indicating hemorrhage
Direct viral invasion of brain
Acute disseminated encephalomyelitis
Periventricular white matter lesions
Cerebral venous thrombosis
Thrombosis of venous system of brain
Severe dehydration and hypotension
Cerebral parenchymal, subdural or subarachnoidal hematoma
Thrombocytopenia, disseminated intravascular coagulation and associated coagulopathies
Vasculitis, infective thromboembolism
Hyperintensities in brainstem and cerebellum
Direct viral invasion
In most cases with dengue virus encephalitis, neuroimaging is normal . Whenever, neuroimaging is abnormal, changes are usually non-specific in terms of etiology but may provide a hint towards the possible pathophysiological process. We report a case highly suggestive of dengue encephalitis with an unusual magnetic resonance imaging finding in brain.
Cortical laminar necrosis is an imaging term classically used to describe high-intensity cortical lesions in a gyriform distribution on T1-weighted magnetic resonance imaging. Cortical laminar necrosis is, pathologically, characterized as necrosis of particular cortical laminae involving neurons, glial cells, and cerebral blood vessels. Cortical laminar necrosis is predominantly caused by hypoxia and metabolic disorders, like hypoglycemia, intoxication, hypoxic-ischemic encephalopathy, renal and hepatic dysfunction, but may also be seen in patients with encephalitis [8–10]. We describe the occurrence of cortical laminar necrosis in a setting highly suggestive of dengue infection, possibly the first instance, in a 15-year-old boy.
Dengue virus was, at large, considered a non-neurotropic virus. With an increase in awareness and improvement in laboratory techniques, dengue virus or dengue viral components are being isolated from brain tissue or from cerebrospinal fluid specimens [11–13]. The mechanism of dengue virus invasion of the central nervous system, and its consequences, is not completely understood. It is supposed that the neurological complications of dengue virus are caused by many mechanisms, singularly or in a combination, like systemic metabolic abnormalities, liver and kidney dysfunctions, direct infection, post-infectious immune disorders, abnormal vascular permeability and flow abnormalities, and coagulopathies. Possibly, the virus enters the central nervous system via infected macrophages. In an experimental study, Bordignon and co-workers noted that a mutant dengue virus-1 escaped immune defense mechanism of the body and caused a severe meningo-encephalitis in mice . In another study that included 84 patients with fatal dengue meningoencephalitis, dengue virus serology in cerebrospinal fluid specimens was positive in 50% of the patients. These findings indicate that dengue virus has neurotropic properties and actively invades central nervous system . Dengue virus-associated cerebrospinal fluid pleocytosis, cerebral parenchymal inflammatory cell infiltrates, and intrathecal synthesis of dengue-specific antibodies also indicate active viral invasion of brain .
A wide variety of neuroimaging changes have been described in dengue encephalitis. Not all patients have neuroimaging abnormalities. Bhoi and co-workers, in a series of 20 patients, noted neuroimaging abnormalities in 50% of patients with dengue virus infection . Multifocal hyperintensities in bilateral periventricular zones, including basal ganglia, may be seen on T2 W and fluid attenuated inversion recovery sequences . Brainstem, cerebellum, corpus callosum and thalamus may also be involved . In many patients with acute hemorrhagic encephalitis, gradient echo sequences reveal patchy areas of blooming suggestive of hemorrhage . In many patients with dengue infection, encephalopathy is being attributed to acute disseminated encephalomyelitis. In such cases, T2-weighted magnetic resonance imaging reveals signal abnormalities in the subcortical and periventricular white matter, with or without gray matter involvement. Dengue-associated acute disseminated encephalomyelitis, being an immune mediated disorder of the central nervous system, has been shown to respond well to intravenous methylprednisolone .
In our case, we kept the possibility of direct dengue virus infection as the foremost cause of cortical laminar necrosis. Metabolic abnormalities, as depicted by mildly deranged liver functions, was kept as the next possibility. As the patient was hemodynamically stable, hypoxic-ischemic insult seems unlikely and the spatial distribution of lesions did not favor the occurrence of posterior leukoencephalopathy. Although evidence of blooming is known in patients with encephalitis, thrombocytopenia in the present scenario might have had a contributory role in the characteristic radiological presentation.
Diagnosis of dengue encephalitis is often not difficult in the setting of a dengue epidemic and a patient presenting with febrile encephalopathy and demonstration of anti-dengue IgM antibodies or dengue genomic material in serum and/or cerebrospinal fluid could be diagnosed as dengue encephalitis . However, in low incidence areas and non-endemic zones, a high index of suspicion should be kept and an effort should be made to rule out other causes of viral encephalitis prevalent in that area . Dengue NS-1 antigen might not be demonstrable in all cases especially those who present after 1 week of onset of symptoms. A low platelet count and altered liver and renal function may help in making diagnosis of dengue encephalitis/encephalopathy.
There is no characteristic neuroimaging finding in dengue encephalitis. Frequently, changes in white matter and deep gray matter have been described. We describe an unusual magnetic resonance imaging finding in a patient dengue encephalitis. Cortical laminar necrosis has never been described in dengue encephalitis.
Availability of data and materials
All the data supporting our findings is contained within the manuscript.
RKG: Case evaluation, contributing in work up of the patient and writing the manuscript. IR: Case evaluation, contributing in work up of the patient and writing the manuscript. RI: Case evaluation, contributing in work up of the patient and writing the manuscript. AJ: Virological evaluation and manuscript review. HSM: Review of literature and writing the manuscript. NK: Review of literature and writing the manuscript. DB: Case evaluation, contributing in work up of the patient and writing the manuscript. All authors read and approved the final manuscript.
On behalf of all authors, the corresponding author states that there is no competing of interest.
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