Expression of chemokine receptors on peripheral blood lymphocytes in multiple sclerosis and neuromyelitis optica

BMC Neurology

Table 1 Combinations of monoclonal antibodies used.

Chemokine receptor-positive cells	Other lymphocyte subsets
FITC-labelled PE-labelled	FITC-labelled PE-labelled
CCR3^c × CD4^b	CD3^a × CD19^a
CD4^b × CCR4^a	CD3^a × CD4^a
CCR5^c ×CD4^b	CD3^a × CD8^a
CXCR3^c × CD4^b	CD4^b × CD25^a
CD8^b × CCR4^a	CD45RA^a × CD4^b
CXCR3^c × CD8^b	CD4^b × CD45RO^a

Monoclonal antibodies were purchased from ^aBD Biosciences, San Jose, CA, USA, ^bBeckman Coulter, Marseille, France, or ^CDako Japan, Kyoto, Japan,
FITC = fluorescein isothiocyanate; PE = phycoerythrin; CCR = CC chemokine receptor; CXCR = CXC chemokine receptor.

ISSN: 1471-2377