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Figure 1 | BMC Neurology

Figure 1

From: Evaluation of the endoplasmic reticulum-stress response in eIF2B-mutated lymphocytes and lymphoblasts from CACH/VWM patients

Figure 1

Expression level of ATF4 protein and four mRNA markers of the ER-stress response in lymphoblasts from eIF2B-mutated patients. (A) ATF4 protein induction (Western-blot); WT: control individuals; (+), (-): with or without 4 h thapsigargin (Tg) treatment. (B-E) Expression levels of ATF4 (B), CHOP (C), ASNS (D) and GRP78 (E) mRNA determined by quantitative real-time polymerase chain reaction; from eIF2B-mutated patients (non-stippled bars), control individuals (stippled bars); under normal conditions (white bars) or following 4 h Tg treatment (grey bars); mean ΔCt values: mean Ct values of genes of interest corrected for the Ct value of the β2M gene.

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BMC Neurology

ISSN: 1471-2377

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