Figure 3

3F4 and 1E4 immunohistochemistry in MV1 sCJD and the proband. 3F4 (A, B, E, F) and 1E4 (C, D, G, H) immunohistochemistry without (A, C, E, G) and with (B, D, F, H) PK pre-treatment in the cerebellum of one case of sCJD MV1 with synaptic PrP deposition (A-D) and in the cerebellum of the proband (E-H) show different patterns, when PK-treatment was performed. In the MV1 sCJD case, synaptic PrP deposition, as revealed with 3F4 and 1E4 antibodies, is observed in the molecular and granular layers of the cerebellum. PrP immunoreactivity is largely resistant to the treatment with PK. Synaptic PrP immunoreactivity with the antibodies 3F4 and 1E4 is also found in the molecular and granular layer in the proband; however, immunostaining is lost following incubation with PK. Notice that formic acid treatment did not seem to modify PK susceptibility.