Figure 4

Immunoblot images under standard conditions. A. 10% brain homogenate digested with 440 ug/ml PK final concentration and incubated with Mab 3F4. M: Molecular weight marker. 1-3: sCJD MM1 thalamus, frontal and temporal cortex, respectively. 4-8: Occipital cortex, cerebellum, parietal cortex, frontal cortex and temporal cortex of the patient, respectively. Film exposure time: 5 minutes. Arrowheads indicate band position. B. 10% brain homogenate digested with decreasing PK concentration: 440 μg/ml (1, 4), 100 μg/ml (2, 5) and 50 μg/ml (3, 6) and incubated with Mab 3F4. Temporal cortex of the patient (1-3) and occipital cortex of a sCJD VV2 case (4-6).