Figure 5

Immunoblot images under modified methodology. A. Immunoblot with TeSeE^® Western kit of patient occipital cortex (1) and sCJD VV2 control (2) incubated with Mab Sha31. B. Immunoblot of TeSeE^® digested and purified samples incubated with Mab 3F4 (upper) and 6H4 (lower). C. PNGase F digestion and detection with Mab 3F4. Film exposure time: 1 minute (upper) and 3 minutes (lower). M: molecular weight marker. 1-8, respectively: occipital cortex, putamen/globus pallidus, cerebellum, parietal cortex, thalamus, frontal cortex, temporal cortex and sCJD VV2 control.