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Figure 7 | BMC Neurology

Figure 7

From: Identification of a novel biomarker candidate, a 4.8-kDa peptide fragment from a neurosecretory protein VGF precursor, by proteomic analysis of cerebrospinal fluid from children with acute encephalopathy using SELDI-TOF-MS

Figure 7

MS analysis of the trypsin digest of the purified protein. The purified protein was digested overnight with trypsin at 37°C and desalted in preparation for Q-TOF MS analysis. Q-TOF analysis was performed using a MALDI-QSTAR. The ions corresponding to the tryptic digest peptides from the 4810 m/z species were selected for subsequent collision-induced dissociation and the resultant fragment ions were analyzed. The resulting MS/MS spectral information was submitted to the Mascot search engine for identification. Among the analyzed peaks, the m/z 1907 peak generated a good fragmentation pattern for the Mascot search.

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