High sensitivity of an ELISA kit for detection of the gamma-isoform of 14-3-3 proteins: usefulness in laboratory diagnosis of human prion disease

Table 4 Analysis of CJD patient s that were not detected in 14-3-3γ protein of WB methods but were beyond the cut-off level (1, 683 AU/ml) in 14-3-3 protein ELISA.

Age	Sex	type	Polymorphism of codon129 in PRNP gene	Mutation of PRNP gene	ELISA of 14-3-3 protein (AU/ml)	Real-time QUIC
69	male	sp	MM	-	2, 101	+
33	female	sp	MM	-	1, 852	+
70	female	sp	MM	-	2, 452	-
73	female	sp	MM	-	2, 531	-
64	male	sp	MM	-	5, 493	+
65	male	fa	MM	E200K	4, 621	+
79	female	sp	VV	-	2, 342	+

sp: sporadic type, ge: genetic type, MM: methioine homozygotes, VV: valine homozygotes, +: positive, -: negative

ISSN: 1471-2377