High sensitivity of an ELISA kit for detection of the gamma-isoform of 14-3-3 proteins: usefulness in laboratory diagnosis of human prion disease

Table 5 Analysis of CJD patient s that were not detected in 14-3-3γ protein of WB methods and were below the cut-off level (1, 683 AU/ml) in 14-3-3 protein ELISA.

Age	Sex	type	Polymorphism of codon129 in PRNP gene	Mutation of PRNP gene	ELISA of 14-3-3 protein (AU/ml)	Real-time QUIC
85	female	ge	MM	V180I	1, 086	-
84	female	ge	MM	V180I	1, 222	-
84	male	ge	MM	V180I	1, 313	-
84	female	ge	MM	V180I	1, 383	-
75	male	ge	MM	M232R	1, 676	+
78	male	sp	MM	-	135	-
66	male	sp	MM	-	1, 224	+

sp: sporadic type, ge: genetic type, MM: methioine homozygotes, VV: valine homozygotes, +: positive, -: negative

ISSN: 1471-2377