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Table 2 PBMC subsets and HLA class I and class II expression

From: Decreased CD8+T cell response to Epstein-Barr virus infected B cells in multiple sclerosis is not due to decreased HLA class I expression on B cells or monocytes

  HC
(n= 59)
All MS
(n= 62)
RRMS
(n= 23)
SPMS
(n= 25)
PPMS
(n= 14)
% CD3+ T cells 72.8 ± 0.8 74.4 ± 1.0 74.2 ± 1.5 72.2 ± 1.7 78.9 ± 1.5
p value   0.216*    < 0.05
% CD4+CD3+ T cells 46.2 ± 1.1 52.2 ± 1.2 49.7 ± 1.9 51.1 ± 1.8 58.1 ± 1.9
p value   < 0.001*   < 0.05 < 0.001
% CD8+CD3+ T cells 23.1 ± 0.8 19.8 ± 0.7 21.7 ± 1.1 18.8 ± 1.0 18.3 ± 1.6
p value   0.007†   < 0.01 < 0.05
CD4:CD8 ratio 2.2 ± 0.1 3.0 ± 0.2 2.5 ± 0.2 3.0 ± 0.3 3.7 ± 0.5
p value   < 0.001†   < 0.01 < 0.001
% NK cells 8.1 ± 0.6 8.1 ± 0.6 9.1 ± 1.1 8.0 ± 1.1 6.5 ± 1.0
p value   0.553†    
% B cells 13.1 ± 0.6 11.7 ± 0.7 11.1 ± 1.1 13.0 ± 1.1 10.5 ± 1.1
p value   0.143*    
% monocytes 7.7 ± 0.6 7.6 ± 0.6 8.2 ± 1.1 8.1 ± 1.0 5.4 ± 1.0
p value   0.752†    
T cell HLA class I MFI 2015 ± 60 2072 ± 46 1964 ± 61 2082 ± 73 2223 ± 107
p value   0.445*    
T cell HLA class II MFI 14.0 ± 0.5 13.7 ± 0.6 13.3 ± 0.8 13.3 ± 0.7 15.0 ± 1.7
p value   0.406†    
B cell HLA class I MFI 3838 ± 132 4063 ± 123 4037 ± 231 3919 ± 164 4362 ± 261
p value   0.214*    
B cell HLA class II MFI 4384 ± 146 4273 ± 153 4313 ± 210 3951 ± 265 4784 ± 315
p value   0.605*    
Monocyte HLA class I MFI 4948 ± 221 4941 ± 189 4823 ± 282 4837 ± 355 5322 ± 297
p value   0.982*    
Monocyte HLA class II MFI 2587 ± 218 2907 ± 236 3053 ± 418 2578 ± 317 3254 ± 561
p value   0.474†    
  1. All values presented as mean ± standard error.
  2. PBMC: peripheral blood mononuclear cells, HC: healthy control subjects, All MS: total group of MS patients, RRMS: relapsing-remitting MS, SPMS: secondary progressive MS, PPMS: primary progressive MS, NK: natural killer, MFI: mean fluorescence intensity.
  3. All p values are for comparisons with HC, using either Student's t test* or the Mann-Whitney rank sum test† for the comparison with All MS, and using one-way analysis of variance with Dunnett's test for multiple comparisons for comparisons with RRMS, SPMS and PPMS; p values < 0.05 are considered significant. In the right three columns of the table, p values are corrected for multiple comparisons using Dunnett's test and are > 0.05 unless otherwise indicated. The CD4:CD8 ratios have been log transformed to make the distributions approximately normal prior to analysis.