Figure 3

Sections from spinal cords of mice 62 days after injection of NMO-IgG (A, D, G), human IgG controls (B, E, H) and vehicle saline controls (C, F, I). Eriochrome staining shows large areas of pale blue demyelination in the NMO-IgG group (A, magnified in D) as indicated by the arrows. Typical EAE areas of posterior column demyelination were present in the control-IgG (B, magnified in E) and vehicle control (C, magnified in F) groups. Aquaporin-4 staining (red) in all groups were similar and appeared increased in areas of demyelination. Scale bars in D-I represent 100 μm.