Figure 4
Quantification and characterization of demyelinated lesions in the spinal cord were based on manual outlines of the total myelinated area (blue) and the demyelinated area (red/yellow) as shown in A. The number of pial lesions greater than 10,000 μm2 made up nearly 40% of all the demyelinated lesions in the NMO-IgG group (B). In addition, the total number of pial lesions in each NMO-IgG mouse on average was higher (C). Accounting for pial lesions of all sizes, the NMO-IgG group had almost double the number compared to the control-IgG and vehicle groups (D).