Table 1 Details of the primary antibody reagents used for immunohistochemical experiments in these studies
Primary antibody | Antigenic target | Concentration | Blocking solution | Manufacturer | Catalog number |
|---|---|---|---|---|---|
Anti-Aquaporin 4, C-terminus | Aquaporin-4 | 1:200 | 5% BSA-PBS + 1:100 NGS + 0.1% Triton X-100 | Millipore | AB3594-200UL |
Purified Rat anti-mouse CD45 | All cells of hematopoietic origin, except erythrocytes | 1:50 | 5% BSA-PBS + 1:100 NGS | BD Pharmingen | 550539 |
Purified Rat anti-mouse CD5 | Thymocytes, T lymphocytes, thymic NK-T cells, subset of B lymphocytes | 1:50 | 5% BSA-PBS + 1:100 NGS + 0.1% Triton X-100 | BD Pharmingen | 553017 |
Purified Rat anti-mouse CD45R (B220) | B lymphocytes | 1:50 | 5% BSA-PBS + 1:100 NGS + 0.1% Triton X-100 | BD Pharmingen | 550286 |
Purified Rat anti-mouse Ly-6G and Ly-6C | Granulocytes | 1:50 | 5% BSA-PBS + 1:100 NGS + 0.1% Triton X-100 | BD Pharmingen | 550291 |
Purified Goat anti-human | Human IgG | 1:250 | 5% BSA-PBS + 1:100 NGS | Invitrogen | A-11013 |