Figure 2

Lentivirus-mediated gene silencing of IFITM3 in glioma cells. (A) Quantitative RT-PCR analysis for IFITM3 mRNA levels in U251, U87-MG and U373 glioma cells; (B) Determination of infection efficiency in the human glioma cells. Representive images of U251 cells after 3 days of lentivirus infection were shown (10× objective lens). (C) Expression analysis of IFITM3 mRNA by qRT-PCR in uninfected U251 cell (Con), cells infected with Lv-shCon, and cells infected with Lv-shIFITM3. The β-actin gene is the internal controls for qRT-PCR. Significant difference from Lv-shCon (P < 0.001). (D) Expression analysis of IFITM3 protein in uninfected group, Lv-shCon group and Lv-shIFITM3 group by western blot.