Figure 3

Lv-sh IFITM3 infection inhibits the proliferation and colony formation of U251 cells. (A) In vitro proliferation assay of U251 cells without infection (Con), cells infected with Lv-shCon, and cells infected with Lv-shIFITM3. Cell proliferation in the Lv-shIFITM3 group was significantly inhibited, as demonstrated by MTT assay. Significant difference from Lv-shCon (P < 0.001). (B) Images recorded under light microscope and fluorescence microscope, representing the size and number of colonies in each group of cells. (C) Statistical analysis of the number of colonies with Giemsa staining. Significant difference from Lv-shCon (P < 0.01).