Figure 5From: Nuclear entrapment and extracellular depletion of PCOLCE is associated with muscle degeneration in oculopharyngeal muscular dystrophy Pcolce protein is sequestered within nuclear aggregates of the mouse OPMD model system. Immunofluorescence staining of TA muscle sections derived from A17.1 OPMD model mice. (A) PABPN1 aggregates were recognised by the high fluorescence intensity of nuclear PABPN1 and their co-localisation with ubiquitin (arrows). Scale bars are 20Â ÎĽm. (B) Co-localisation of Pcolce with PABPN1 was conducted on TA muscle sections from 12, 18 and 26Â week old A17.1 mice. PABPN1 is visualised with 3Â F5-VHH and mouse anti-myc antibodies (green), Pcolce or ubiquitin are visualised with rabbit antibodies and are marked in red. Co-localisation between Pabpn1 and Pcolce is shown in yellow in the merged image. The nucleus is counterstained with DAPI (blue). Nuclear aggregates are indicated with arrows. Scale bars equal 10Â ÎĽm. Immunostaining was performed on sections from 3 mice. Shown are representative images.Back to article page