PCOLCE sub-cellular localisation in OPMD and control muscle biopsies. Muscle cryosections were co-stained for PABPN1 (red; 3 F5-VHH) and PCOLCE (green; 3 F5-VHH). Nuclei were counterstained with DAPI (blue). Arrows point to nuclear PCOLCE that colocalizes with aggregated PABPN1. (A) The sarcolemma is highlighted by staining for dysferilin (purple). Scale bar equals 10 μm. (B) Immunofluorescence after KCl treatment. Treatment with 1 M KCl disturbs localisation of soluble proteins whilst aggregates remain intact (arrow). Co-localisation between PABPN1 and PCOLCE is shown in the merged image. Scale bar equals 5 μm. (C) Changes in PCOLCE sub-cellular localisation shown at low magnification. PABPN1 and DAPI mark nuclei. Scale bar equals 30 μm.