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Table 1 Oligonucleotide primer used in the amplification of GAD1 exons.

From: Homozygosity for a missense mutation in the 67 kDa isoform of glutamate decarboxylase in a family with autosomal recessive spastic cerebral palsy: parallels with Stiff-Person Syndrome and other movement disorders

Primer

Forward

Reverse

Exon 1

dGCCCCATTTATTTCCCAGCC

dGCACAGCTCTCGCTTCTCTT

Exon 2

dGAAAACCATTGTCCTCCACC

dGCCTGTCGGCTCACAGATT

Exon 3

dACCAGCTTCTTGTGCCATAG

dATCTACTGGCTAGCATGGGG

Exon 4

dATTCCATGTCTGAGCAGCCT

dACTGTTACTGCCCAAGCTTG

Exon 5

dGCCGTTTGCCTTCAAGATAG

dAGAACCACTGGGACTGAACT

Exon E

dACCAGTATCTCCTCGCCATG

dTTGGGAGGCCCCTGGAAATT

Exon 6

dACCCAACTACAAATACTAAACC

dAATAGGAAGTCAGGGTATCC

Exon 7

dGAGACACCAGCTCAGCGTTC

dCTGCAACAAACAGAGGCTCG

Exon 8

dGTCGGGGATGCTTTCTCCATG

dCTCAGTACATTGTGCCAAGC

Exon 9

dCAAGCTGCTAATGGTCTGTT

dGTCTCATATTATCAAGGACTG

Exon 10

dCACAATTCTTCTTCCTGTGA

dTGGGGAGGAGCTTGAGGCAA

Exon 11

dACAATCAGTGTGGGCTGAAC

dGAAGCAAACTTAGACCGAAA

Exon 12

dCTTGAGTTGGAATGGGTGTT

dACTGCAAAGAGACCCCACGT

Exon 13

dTCCTTCCAAGCAGCCTAGTT

dGTGATATATCTTTGCCCCTC

Exon 14

dGACAGCATAGCCTTCCCAAA

dCATGTTGCCAGAAGCTTCAG

Exon 15

dGGTTTGGGAACAGCTTTCTC

dTTCCCCCACTAGAAAGGCAC

Exon 16

dGTTAAAAAGAGAGGGTGTTC

dCCCTCAATGAAATGGCCTGT

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BMC Neurology

ISSN: 1471-2377

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