The percentage of cells that incorporated BrdU decreased with increased age and was lower in Lama2
-/- muscles, but higher in mdx muscles, than in normal control muscles. A. The percentage of the total population of mononucleate muscle cells that incorporated BrdU was decreased as postnatal development proceeded. In muscles of two control strains (C57Bl/6J, the control for Lama2
-/- muscles; and C57BL/10ScSn, the control for mdx muscles), there was a similar several-fold decrease in BrdU incorporation from 3–4 weeks to 7.5 weeks after birth, which is consistent with the slowing of muscle growth found during this period. B. The percentage of all cells that incorporated BrdU was significantly lower in 3 week old Lama2
-/- muscles than in normal control muscles of the same age. In contrast, the percentage of all that incorporated BrdU was signifcantly higher in 4 week old and 7.5 week old mdx muscles than in normal control muscles of the same age. Error bars represent standard deviation with n = 3. * indicates diseased and healthy control samples differ significantly at P < 0.05.