Table 1 Primers utilized for real-time RT-PCR analysis
Genes | GenBank accession No. | Sense primers | Antisense primers | PCR product (bp) |
|---|---|---|---|---|
ISG15 (G1P2) | NM_005101 (M13755) | 5'aagcccctgagcaccgtgttcat3' | 5'ttgatcctgctcggatgctggtg3' | 102 |
SCYB10 (CXCL10, IP-10) | NM_001565 (X02530) | 5'tcgatgcagtgcttccaaggatgg3' | 5'ccttcctacaggagtagtagcagc3' | 162 |
SCYA8 (CCL8, MCP2) | NM_005623 (Y10802) | 5'tctgtgctgaccccaaggagagat3' | 5'taatgtcacactgcacctggggga3' | 164 |
SCYA2 (CCL2, MCP1) | NM_002982 (S71513) | 5'ctagctttccccagacaccctgtt3' | 5'ccaggggtagaactgtggttcaag3' | 197 |
SCYB2 (CXCL2, GRO2) | NM_002089 | 5'cccgcatcgcccatggttaagaaa3' | 5'tcttctgttcctgtaaggcagggc3' | 131 |
FOS | NM_005252 | 5'gagctggtgcattacagagaggag3' | 5'ggacttgagtccacacatggatgc3' | 140 |
RGS14 | NM_006480 | 5'tgacagctacccaacagtccagga3' | 5'agggattgggggtgagcttgttga3' | 222 |
G3PDH | NM_002046 | 5'ccatgttcgtcatgggtgtgaacca3' | 5'gccagtagaggcagggatgatgttc3' | 251 |