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Table 3 Top 20 downregulated genes in PBMC following exposue to IFNβ

From: Microarray analysis identifies a set of CXCR3 and CCR2 ligand chemokines as early IFNβ-responsive genes in peripheral blood lymphocytes in vitro: an implication for IFNβ-related adverse effects in multiple sclerosis

No. Cy5/Cy3 ratio Symbol GenBank Gene name
at 3 hours
1 0.29 RGS14 NM_006480 regulator of G-protein signaling 14
2 0.30 FOS NM_005252 cellular oncogene c-fos
3 0.31 SCYB2 NM_002089 chemokine (C-X-C motif) ligand 2 (GRO2, MIP2A)
4 0.41 PPARG2 U63415 peroxisome proliferative activated receptor gamma, isoform 2
5 0.41 TNFSF11 NM_003701 tumor necrosis factor ligand superfamily, member 11 (RANKL), isoform 1
6 0.42 ABCC4 NM_005845 ATP-binding cassette, subfamily C, member 4 (MRP4)
7 0.42 MERTK NM_006343 c-mer proto-oncogene tyrosine kinase
8 0.42 TGFBR3 NM_003243 transforming growth factor beta receptor III (betaglycan)
9 0.44 ATP2B1 J04027 ATPase, Ca++ transporting, plasma membrane 1, isoform 1b
10 0.45 SCYB1 NM_001511 chemokine (C-X-C motif) ligand 1 (GRO1, MGSA)
11 0.45 IL8 NM_000584 interleukin 8 (SCYB8, CXCL8)
12 0.45 TRAF5 NM_004619 TNF receptor-associated factor 5, variant 1
13 0.47 CD3G NM_000073 CD3G antigen, gamma polypeptide
14 0.47 IL1A NM_000575 interleukin 1 alpha
15 0.47 TCFL5 NM_006602 transcription factor-like 5
16 0.48 LAT AF036905 linker for activation of T cells, isoform b
17 0.48 HNMT U08092 histamine N-methyltransferase, isoform 1
18 0.48 SLC3A1 NM_000341 solute carrier family 3, member 1
19 0.49 MGST1 NM_145764 microsomal glutathione S-transferase 1, variant 1d
20 0.49 TNFSF8 NM_001244 tumor necrosis factor ligand superfamily, member 8 (CD30L)
at 24 hours
1 0.18 ABCC3 AF083552 ATP-binding cassette, subfamily C, member 3, isoform MRP3
2 0.20 FOS NM_005252 cellular oncogene c-fos
3 0.30 ALDH2 NM_000690 aldehyde dehydrogenase 2 family
4 0.30 TPST1 NM_003596 tyrosylprotein sulfotransferase 1
5 0.31 CD9 NM_001769 CD9 antigen
6 0.34 NRG1 NM_013962 neuregulin 1, isoform GGF2
7 0.35 TLR5 NM_003268 toll-like receptor 5
8 0.36 PTGS1 NM_000962 prostaglandin-endoperoxide synthase 1 (COX1), isoform 1
9 0.37 MGST2 NM_002413 microsomal glutathione S-transferase 2
10 0.38 ITGAM NM_000632 integrin, alpha M (CD11B, MAC-1, CR3A)
11 0.38 SCYA24 NM_002991 chemokine (C-C motif) ligand 24 (eotaxin 2)
12 0.38 CYP27A1 NM_000784 cytochrome P450, family 27, subfamily A, polypeptide 1 (CTX)
13 0.39 ALDH1A1 NM_000689 aldehyde dehydrogenase 1 family, member A1
14 0.39 MYCL1 NM_005376 v-myc myelocytomatosis viral oncogene homolog 1 (LMYC)
15 0.41 ATP6AP2 NM_005765 ATPase, H+ transporting, lysosomal accessory protein 2 (ATP6M8-9)
16 0.44 PRKCG NM_002739 protein kinase C gamma
17 0.45 ATP2B1 J04027 ATPase, Ca++ transporting, plasma membrane 1, isoform 1b
18 0.46 AP1S2 NM_003916 adaptor-related protein complex 1, sigma 2 subunit
19 0.47 NR6A1 NM_001489 nuclear receptor subfamily 6, group A, member 1 (RTR), isoform 2
20 0.47 GCG NM_173916 glucagon
  1. Top 20 downregulated genes in PBMC of the subject #1 (a 46 year-old healthy man) by exposure to 50 ng/ml recombinant human IFNβ for 3 or 24 hours are listed with Cy5/Cy3 signal intensity ratio, gene symbol, GenBank accession number, and gene name.