Figure 4From: Significant difference between three observers in the assessment of intraepidermal nerve fiber density in skin biopsy Skin biopsy immunostained for PGP 9.5, which were evaluated in this study. Number of IENF stated by the observers (MF, ISH and SW) of this study: 0–2 fibers. The different results were probably caused by difficulties to determine the correct position of the fiber: (e.g.: the fiber approaches the basement membrane but do not cross it → 0 fibers, the fiber branches after crossing the basement membrane → 1 fiber, the fiber branches within the basement membrane → 2 fibers.Back to article page