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Table 1 PCR-conditions and sequences of H1-SNP-specific primers.

From: Role of the H1 haplotype of microtubule-associated protein tau (MAPT) gene in Greek patients with Parkinson's disease

Primer Sequences PCR-conditions
H1 F
H1 R
5'-GGA AGA CGT TCT CAC TGA TCT G-3'
5'-AGG AGT CTG GCT TCA GTC TCT C-3'
95°C 5 min, 35× (95°C 30s, 55°C 30s, 72°C 30s), 72°C 10 min
rs242562 F
rs242562 R
5'-GGC GAT TCC GCT GAG TCA CC-3'
5'-GGC CCT GCT GGA GTC AAG AG-3'
95°C 5 min, 35× (95°C 30s, 62°C 30s, 72°C 30s), 72°C 10 min
rs2435207 F
rs2435207 R
5'-CTG AGG GCC GTC ACT GTC TG-3'
5'-CCT CAA GCC CAT TCT CTG AC-3'
95°C 5 min, 35× (95°C 30s, 58°C 30s, 72°C 30s), 72°C 10 min
  1. F, forward; R, reverse; SNP, single-nucleotide polymorphisms.