Transient SOX4 overexpression inhibits growth of LN229 cells and induces G0/G1 cell cycle arrest. (A) LN229 cells were transiently transfected with Flag-Sox4-HA or pEGFP-N1 plasmid as control. After 48 hours, protein expression was assayed by Western blotting using anti-HA antibody (B) Cell vitality was determined by CCK-8 assay after transfection of LN229 cells with Flag-Sox4-HA plasmid or pEGFP-N1 and Cre-GFP as control at the indicated time points. Values at the indicated time points were provided as the mean cell number with an SD of eight wells. *, P < 0.05 (C) Colonies formed by Flag-Sox4-HA or pEGFP-N1or Cre-GFP infected LN229 cells were shown 2 weeks after plating in 6 well plate. Upper panel showed the quantification of the colony in Flag-Sox4-HA or pEGFP-N1or Cre-GFP infected LN229 cells. Values are the means ± SD of triplicate experiments, *P < 0.05 (D) Impact of SOX4 on cell cycle of LN229 cells. The percentage of cells in G0/G1, S, and G2/M phases is shown in the left panel. And the statistic analysis is also shown in the right panel.