Overexpression of SOX4 reduces proliferation and colony formation of GBM cells. (A) Validation of SOX4 expression in stable SOX4 overexpression cells (LN229_pSFH/A172_pSFH/U87_pSFH) and control cells (LN229_con/A172_con/U87_con) by Real-time PCR and Western blot with anti-HA antibody. (B) The vitality of GBM cells stably express Flag-SOX4-HA or GFP was determined by CCK8 assay. Values at the indicated time points were provided as the mean cell number with an SD of eight wells. *, P < 0.05 (C) Colonies formed by LN229_pSFH/A172_pSFH or LN229_con/A172_con were shown 2 weeks after plating in 6 well plate. Right panel showed the quantification of the colony formation. Values are the means ± SD of triplicate experiments. *, P < 0.05 (D) Colonies formed by U87_pSFH or U87_con were shown 3 weeks after plating in Soft agar.