Knock out SOX4 by Cre recombinase restored cell growth and colony formation. (A) Validation of SOX4 expression in Flag-SOX4-HA stable expressed cells transfected with Cre-GFP or pEGFP-N1 by Real-time PCR and Western blot with anti-HA antibody (B) Cell vitality was determined by CCK-8 assay after transfection of Flag-SOX4-HA stable expressed cells with Cre-GFP or pEGFP-N1 as control. Values at the indicated time points were provided as the mean cell number with an SD of eight wells. *P < 0.05 (C) The reduction of SOX4 reduced cells accumulated in G0/G1 phase. The percentage of cells in G0/G1, S, and G2/M phases is shown in the left panel. And the statistic analysis is also shown in the right panel.