Figure 3
From: Creutzfeldt-Jakob disease mimicking autoimmune encephalitis with CASPR2 antibodies
Frontal cortex (lower side towards pia mater; upper side towards white matter), PrP; Scale bar 100 um. Note the confluent spongiosis and the diffuse synaptic PrP deposits. Brains were fixed in 4% formalin and paraffin-embedded tissue samples of frontal cortex were cut into 3 μm thick serial sections, mounted on glass slides and processed for immunhistochemical staining using specific antibodies to PrP. Visualization of primary antibody was achieved using the diaminobenzidine streptavidin-biotin horseradish peroxidase method on an automated stainer (Ventana/Roche).