Figure 4

Neuritic profiles inside and surrounding the wall of intracerebral arterioles from cases with brain amyloid pathology. Panels (A-C) show a partial colocalization of BACE1 and synaptophysin immunofluorescence in the vascular wall of an arteriole in the subcortical white matter (WM) (arrows pointing to colocalized parts), with a nearby arteriole (circled with broken purple line) exhibited background signal for both markers. Panels (D) shows BACE1 labeled dystrophic neurites (arrows) partially colabeled (enlarged insert) for nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) surrounding an intracortical vessel, with clusters of dystrophic neurites (DN) in the field. Panel (E) shows NADPH-d positive dystrophic neurites (arrows) in and around the wall of a vessel with Aβ deposits. Panel (F) shows rosette-like NADPH-d positive dystrophic neurites in compact amyloid plaques (NP). Panels (G,H) show BACE1 labeled neurites in small (G) and larger (H) amounts in the vascular wall that are partially colabeled by Prussian blue, as are those organized as rosset-like clusters (I). The colabeled parts appear grey to black because of the brown DAB background color. The AOI label in (A) illustrates a method to measure optic density in normal arterioles (referring to Figure 3K-L). Scale bar = 100 μm in (A) applying to (B-I).