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Table 3 Immunological Assays

From: Assessment of immune functions and MRI disease activity in relapsing-remitting multiple sclerosis patients switching from natalizumab to fingolimod (ToFingo-Successor)

 

Biomaterial

Assay

PBMC1

PB Immune cells2

CSF2

Flow cytometric assessment of CD49d and CD62L expression

X

  

In vitro transmigration across BBB

X

  

VZV-specific T-cell response

X

  

Immune phenotyping via flow cytometry

X

X

X

  1. 1purified via density gradient centrifugation and cryo-preserved
  2. 2freshly obtained, treated with erythrocyte-lysis buffer