Fig. 3

The effect of high-dose simvastatin on expression of HIF-1α and BACE. Cells were incubated with 0 and 10 μM simvastatin under hypoxia. Intracellular HIF-1α and BACE levels were measured by western blotting. a In SAD cybrids, 10 μM simvastatin did not influence HIF-1α. b In SAD cybrids, BACE significantly increased at 6 h and 12 h in the presence of 10 μM simvastatin (*P < 0.05). c In CTL, HIF-1α significantly increased at 3 h, 6 h, and 12 h in the presence of 10 μM simvastatin (*P < 0.05). d In CTL, BACE significantly increased at 3 h in the presence of 10 μM simvastatin (*P < 0.05). X-axis represents hypoxia duration; Y-axis represents represents percent values from the immunoassay versus the 0 time point. All experiments were repeated three times