Fig. 5

Suppressive function of the induced CD4⁺CD25⁺ T cells on the proliferation of autologous CD4⁺CD25ˉ T cells in MG patients. a The induced CD4⁺CD25⁺ T cells were co-cultured with autologous CD4⁺CD25ˉT cells at a 1:1 ratio to assay their suppressive function on the proliferation of CD4⁺CD25ˉ T cells. Results are expressed as mean cpm ± SD, n = 16. ^*, P < 0.05 vs. CD4⁺CD25ˉ T cells cultured alone. &, P < 0.05 vs. co-cultures of CD4⁺CD25ˉT cells and nTregs. ^#, P < 0.05 vs. 40 ng/mL induced CD4⁺CD25⁺ T cells. b IFN-γ dose-dependent induction of FoxP3 mRNA expression and the suppressive percentage of the induced CD4⁺CD25⁺T cells in suppressing proliferation of co-cultured autologous CD4⁺CD25ˉT cells. There is linear regression between them (r² = 0.917, p = 0.029)