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Fig. 2 | BMC Neurology

Fig. 2

From: Chloroquine, an autophagy inhibitor, potentiates the radiosensitivity of glioma initiating cells by inhibiting autophagy and activating apoptosis

Fig. 2

Combination treatment of irradiation and chloroquine obviously promoted apoptosis in U87 GIC. a U87 GIC were treated with X-ray(6Gy) alone and chloroquine(20 μM) alone or in combination for 24 h, and the promotion of apoptosis was measured using AnnexinV-FITC/PI double staining. b Quantitative analysis of apoptotic cells. Data are demonstrated as the mean ± SD,*, p < 0.05, **p < 0.01, n = 3. c Apoptosis -associated proteins were investigated by Western blotting. d Relative density of Bcl-2 and Cleaved caspase-3 versus GAPDH in each sample measured by densitometry of the blots. Densitometric analysis of the immunoblot is expressed as a percentage of control. Data are presented as the mean ± SD, *, p < 0.05 versus control, **p < 0.01, versus control, n = 3

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BMC Neurology

ISSN: 1471-2377

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