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Table 1 Experiments completed for each study (i.e. set of animals)

From: Sex differences underlying orofacial varicella zoster associated pain in rats

Study Experiment Groups Number of animals (male Sprague Dawley rats unless otherwise indicated)
Study #1 Thermal and mechanical hypersensitivity assay (Figure 3). Neurite retraction study using PGP 9.5 (n = 3/group randomly selected, Figure 2). Mewo (control) Poka (VZV) n = 10 n = 10
Study #2 Place escape/avoidance paradigm (Figure 4). VZV infection detected with IE62 marker (Figure 1). PBS (vehicle) Mewo (control) Poka (VZV virus) n = 6 n = 6 n = 6
Study #3 Gabapentin assay (Figure 5). Mewo/vehicle Mewo/gabapentin Poka/vehicle Poka/gabapentin n = 5 n = 5 n = 5 n = 5
Study #4 Sex differences study comparing males and females (Figure 6). Mewo/female Mewo/male Poka/female Poka/male n = 24 n = 7–15 n = 25 n = 7–15
Study #5 OVX females as compared to males (Sprague Dawley and Long Evans, Figure 7). VZV infection detected with IE62 marker (Sprague-Dawley rats, no figure). Mewo/male Mewo/female Mewo/OVX female Poka/male Poka/female Poka/OVX female n = 5 n = 4 n = 4 n = 7 n = 6 n = 6 n = 5 (Long Evans) for all treatment groups
Study #6 Female estrous cycle study (Figure 8). Mewo diestrus Mewo proestrus/estrus Poka diestrus Poka proestrus/estrus n ≥ 8 n ≥ 8 n ≥ 8 n ≥ 8