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Table 1 Sequences of primers (5′ → 3′) used to construct plasmids encoding various human GFAP alleles

From: Aggregation-prone GFAP mutation in Alexander disease validated using a zebrafish model

Allele

Sequences

WT

Forward: TAGTAGGATCCATGGAGAGGAGACGCATCAC

Reverse: TAGTCGATATCATCATCACATCCTTGTGCTCCTGCTTG

p.Arg79Cys

Forward: GAGATGATGGAGCTCAATGACtGCTTTGCCAGCTACATCGAG

Reverse: CTCGATGTAGCTGGCAAAGCaGTCATTGAGCTCCATCATCTC

p.Arg79His

Forward: GAGATGATGGAGCTCAATGACCaCTTTGCCAGCTACATCGAG

Reverse: CTCGATGTAGCTGGCAAAGtGGTCATTGAGCTCCATCATCTC

p.Asp128Asn

Forward: GAGAGCTGCGGCTGCGGCTCaATCAACTCACCGCCAACAG

Reverse: CTGTTGGCGGTGAGTTGATtGAGCCGCAGCCGCAGCTCTC

p.Asp157Asn

Forward: GCAGAAGCTCCAGaATGAAACCAACCTG

Reverse: CAGGTTGGTTTCATtCTGGAGCTTCTGC

p.Arg239Cys

Forward: CAGCCCTGAAAGAGATCtGCACGCAGTATGAGGCAATG

Reverse: CATTGCCTCATACTGCGTGCaGATCTCTTTCAGGGCTG

p.Arg239His

Forward: CAGCCCTGAAAGAGATCCaCACGCAGTATGAGGCAATG

Reverse: CATTGCCTCATACTGCGTGtGGATCTCTTTCAGGGCTG

  1. Lower case indicates mutated nucleotides