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Fig. 4 | BMC Neurology

Fig. 4

From: Altered development of dopaminergic neurons differentiated from stem cells from human exfoliated deciduous teeth of a patient with Down syndrome

Fig. 4

Aberrant expression of molecules involved in DA homeostasis in DS-DN. a, b The mRNA expression of DAT1 (a) and VMAT2 (b) was measured by RT-qPCR. The relative expression of each gene was calculated with the 2-ΔΔCt method. Graphs show the mean ± SEM from four experiments. *P < 0.05. c-e NMDAR1 and TH in Ctrl- and DS-DN cells were observed by immunofluorescence microscopy (c). Cells were counterstained with DAPI. Scale bar = 25 μm. Details of the boxed region in (c) are shown (d). Scale bar = 5 μm. The number of NMDAR1 puncta per 25 μm of neurite was counted (e). Graph shows the mean ± SEM of NMDAR1 puncta from 30 neurites. ***P < 0.001

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