Study protocol: ASCRIBED: the impact of Acute SystematiC inflammation upon cerebRospinal fluId and blood BiomarkErs of brain inflammation and injury in dementia: a study in acute hip fracture patients

Table 1 Sample and data collection schedule

Timepoint	Admission/Pre-Op Period	Day of Operation	Post-Op Period
Timepoint	Admission/Pre-Op Period	Day 0	48 (± 4 hours) post-op	Time 1 (1 month ± 5 days)
Consent/ Agreement	X		X^a
AMT and/or 4AT	X		X^b
Collection of blood EDTA sample (6 ml)		X
Collection of blood serum clotted sample (10 ml)		X	X^b
Collection of Cerebrospinal fluid (CSF) sample (≥ 2.0 ml)		X
MMSE~ 2: SV			X^b
IQCODE (To be completed by the suitable informant)			X^c
Evidence of dementia from patient’s medical/GP records				X^c
Medication information				X^c
Collection of blood PAXgene RNA sample (2.5 ml)		X

^aTaken if patient’s capacity status has changed from pre-operative time period (Eng. only);
^bShould the time window be unworkable, research nurses will collect MMSE~ 2: SV data and bloods at the next earliest opportunity but not beyond 60 h post-op;
^cCan be gained at any point before the 1-month (± 5 days) time period elapses

ISSN: 1471-2377