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Table 1 PCR detection of JCV DNA in CSF specimens from patients suspected of having PML

From: Improving detection of JC virus by ultrafiltration of cerebrospinal fluid before polymerase chain reaction for the diagnosis of progressive multifocal leukoencephalopathy

  Standard procedure Ultrafiltration procedurea
Patient Positive reactionb Copies / reactionc Copies/mL CSFd Positive reaction Copies / reactione Copies / mL CSFf
1 3/3 207,033 5,175,825 3/3 1,030,133 3,219,166
2 3/3 766 19,150 3/3 2433 7603
3 3/3 321 8025 3/3 1383 4322
4 3/3 123 3075 3/3 568 1775
5 3/3 20 500 3/3 110 344
6 3/3 12 300 3/3 93 291
7 3/3 7 175 3/3 61 191
8 1/3 NA NA 3/3 6 19
9 0/3 NA NA 0/3 NA NA
10 0/3 NA NA 0/3 NA NA
11 0/3 NA NA 0/3 NA NA
12 0/3 NA NA 0/3 NA NA
13 0/3 NA NA 0/3 NA NA
14 0/3 NA NA 0/3 NA NA
15 0/3 NA NA 0/3 NA NA
16 0/3 NA NA 0/3 NA NA
17 0/3 NA NA 0/3 NA NA
18 0/3 NA NA 0/3 NA NA
19 0/3 NA NA 0/3 NA NA
20 0/3 NA NA 0/3 NA NA
  1. Abbreviations: CSF Cerebrospinal fluid, JCV JC virus, NA Not applicable, PCR Polymerase chain reaction, PML Progressive multifocal leukoencephalopathy
  2. aEach CSF specimen was concentrated from 4 mL to 500 μL by ultrafiltration before DNA extraction
  3. bThe PCR was repeated three times
  4. cThe mean copy numbers of JCV DNA in 2-μL PCR template of total 25-μL DNA extract from 500-μL CSF were determined, when all three PCRs were positive
  5. dThe copy numbers of JCV DNA per mL were calculated with respect to total volume of DNA extracts (12.5-fold) and the amounts of test samples (2-fold)
  6. eThe mean copy numbers of JCV DNA in 2-μL PCR template of total 25-μL DNA extract from 4-mL CSF were determined, when all three PCRs were positive
  7. fThe copy numbers of JCV DNA per mL were calculated with respect to total volume of DNA extracts (12.5-fold) and the amounts of test samples (0.25-fold)