Fig. 2

Photomicrographs of original brain and metastatic spinal glioblastoma multiforme (GBM). a–c Hematoxylin and eosin staining demonstrating high-grade glioma featuring nuclear atypia, cellular pleomorphism, mitotic activity, and remarkable necrosis. d–f The tumor cells were determined to exhibit glial fibrillary acidic protein (GFAP) positivity. g–i Ki-67 proliferative index was high (approximately 35%) initially; it was approximately 20% after proton beam radiotherapy and approximately 30% after spinal metastasis. Original magnification, 200×