Fig. 1

Undiluted CSF antibodies binding to NMDA-NR1 subunit-transfected HEK293T cells (Autoimmune encephalitis mosaic-6, EUROIMMUN, Lübeck, Germany) detected using fluorescein isothiocyanate-conjugated human IgG. a CSF positive (scored 2) × 20; b CSF positive (scored 1) × 40; c CSF positive (scored 2) × 40; d positive commercial control × 40; e CSF negative (scored 0). Immunofluorescence intensity was scored as 2 (=positive control); as 1 for low positive; and as 0 for negative. NMDAR = N-methyl-D-aspartate receptor, HEK = Human embryonic kidney