Gliosarcoma vs. glioblastoma: a retrospective case series using molecular profiling

BMC Neurology

Table 1 Methods used for molecular profiling, sorted by number of tests

Abbreviation	Method	Molecule	n	Examples
				NSD	From Table 3
NGS	next generation sequencing	DNA	599	PTEN, TP53	IDH1, IDH2, PTCH1
CNA	copy number amplification	DNA	444	ALK, BRAF	CALR, LYL1, EGFR
F-RNA	fusion gene present	RNA	54	EGFRvIII, BRAF	NTRK1
IHC	immunohistochemistry	protein	28	ALK, EGFR	PD-1, PD-L1
SS	Sanger sequencing	DNA	9	BRAF1, KIT
FISH	fluorescence in-situ hybridization	DNA	6	del(1p/19q), HER2/neu
CISH	chromogenic in situ hybridization	DNA	5	EGFR, HER2/neu
RFLP	restriction fragment length polymorphism	DNA	3	EGFR T790M
FA	fragment analysis	DNA	2		EGFRvIII
FV	fusion variant	RNA	2	EGFR, MET
FFA	fusion or fragment analysis		1		EGFRvIII
HS	H score (immunohistochemistry score)	protein	1	EGFR
P	pyrosequencing	DNA	1	MGMTpm status
MSI	microsatellite instability	DNA	1

Some of these abbreviations are used in Tables 3 and 5. Examples of DNA/ RNA/ proteins assessed are given for each method
Abbreviations: n Number of tests using this method, NSD Test was not significantly different (GS vs. GB), MGMTpm MGMT promoter methylation

ISSN: 1471-2377