Fig. 6

Double immunohistochemical evaluation using antibodies against TDP-43 and p62. To evaluate whether each cytopathology (ALS and NIID pathology) may coexist in a motor neuron, a double immunofluorescence study was performed using antibodies against non-phosphorylated TDP-43 and p62. The photographs (a-c) show a normal motor neuron with preservation of native TDP-43 in the nucleus (arrow). The photographs (d-f) demonstrate a motor neuron with skein-like inclusion (arrowhead), both positive for TDP-43 and p62, along with loss of nuclear native TDP-43 (punched-out arrow). The photographs (g-i) showing p62-positive inclusion (thin arrow) in the nucleus of a motor neuron with remaining native TDP-43 (arrow) in the nucleus. a, d, g TDP-43, b, e, h p62 and c, f, i merge. Scale bars = 20 μm