Fig. 2

Histopathology. Diffuse marked cerebral atrophy (a, b) and depigmentation of the substantia nigra (c) and locus ceruleus (d) are shown. Post-mortem tissue staining of the frontal cortex (e, g), cerebellar cortex (f), Edinger–Westphal nucleus (h), substantia nigra (i), and cornu ammonis 2 (j) is also shown. (e, f, h) Hematoxylin and eosin staining; (g) prion immunostaining; (i, j) phosphorylated α-synuclein immunostaining. Scale bars: a–d, 1 cm; e–g, j, 50 μm; h, i, 20 μm)