Table 1 Characteristics of primers used for examining the genes
From: The role of Bordetella pertussis in the development of multiple sclerosis
Primers | Sequences (5′ → 3′) | Target | Size (bp) | Tm (°C) |
|---|---|---|---|---|
F1-BP | TCCGAACCGGATTTGAGAAA | IS481 | 60 | 78.5 |
R1-BP | CCGGGCTCCTTGAGTGAA | |||
F1-BpP | ATGCTGGATCGCAAGTTGATG | IS1001 | 81 | 85.8 |
R2-BpP | TGGGTCTTCGGGCCATT | |||
F1-16S-TQM | CGTGTCGTGAGATGTTGGGTTA | 16S rDNA | 60 | 78.5 |
R1-16S-TQM | GACGTCATCCCCACCTTCCT |