Fig. 3

ALS serum pre-treatment did not influence pericyte intracellular expression of MCP-1 or VCAM-1 in response to pro-inflammatory compounds. A Representative images of ALS or control serum pre-treated pericytes 8 hours after vehicle or different pro-inflammatory stimuli were applied (IL-1β, IFN-γ or TNF-α). Scale bar = 50 μm. Inflammatory response is represented by an upregulation of MCP-1 and VCAM-1. B Quantification of percentage cells positive for MCP-1, mean +/− SEM. C Quantification of mean integrated intensity of VCAM-1 per positive cell, mean +/− SEM