Fig. 4

ALS serum pre-treatment did not significantly alter pericyte cell number. A There was a consistent but non-significant increase in ALS serum pre-treated cell nuclei observed across all pro-inflammatory stimuli and vehicle. Values are average cell counts per 10X magnification image site (equivalent to 1393.2 × 1393.2 μm). Two sites were imaged per well for each of the 15 ALS and 15 control sera-treated cell samples across all pro-inflammatory stimuli conditions and vehicle. Results are plotted as mean +/− SEM. B Overall combined cell nuclei count (from all treatments). Statistical analysis determined by Mann-Whitney test