Fig. 3

CD4 ⁺ CD25 ⁺ T cell populations from migraineurs were significantly lower than controls. (A) The lymphocytes and monocytes populations are highlighted, and the single cell population was selected only. CD3⁺ and CD4 ⁺ population were selected, respectively. Finally, CD4⁺ CD25⁺ population was highlighted. (B) CD4⁺ CD25⁺ T cell populations from migraineurs were significantly lower than controls (5.66 ± 1.35 vs. 8.29 ± 2.31), **P = 0.001. Data are represented as means ± SD, significance was determined by nonparametric Wilcoxon signed rank test