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Table 2 Conditions for the real-time polymerase chain reaction (RT-PCR).

From: Association between miRNA-145 and miRNA-155 expression in peripheral blood mononuclear cells of patients with multiple sclerosis: a case-control study

Step

Tm

Time

Cycle namber

Pre-incubation

94c

600s

1

Denaturation

94c

10s

45

Annealing and Extension

60c

60s

Melting