Table 2 Overview of the assessments conducted at specified visits (optional parts are given in brackets)

medical history & examination

 demographics

x

x

x

(x)

 medical history

diagnosis,

attack/relapse history, comorbidities, current symptoms and complaints, dietary and lifestyle factors

x

x

x

x

 therapies

disease modifying therapy, relapse therapy, full drug list, supportive therapies

x

x

x

x

 neurological examination

EDSS

SARA

x

x

x

x

x

(x)

(x)

biospecimens

 blood samples

• biobanking

3 heparin (9ml each),

3 serum (10ml each),

3 EDTA (3,6 and 9 ml each),

1 PAX (2,5ml each).

• clinical routine parameters

• antibody testing

x

x

x

(x)

 stool samples

standard 16 S rRNA sequencing, viability of bacteria, analysis of microbial metabolites

x

x

x

(x)

 optional consent

use of biospecimens from clinical routine (cerebrospinal fluid, biopsy, plasma from plasmaphereses)

(x)

nutrition and lifestyle

 body height and weight

BMI

x

x

x

(x)

 body composition (BIA)

body fat mass, fat-free mass, total body water, body cell mass, extracellular mass

x

x

x

(x)

 vital signs

blood pressure, heart rate

x

x

x

(x)

 dietary habits and practice of stress-reducing behavior

FFQ and HOLISM life-style assessment

x

x

x

(x)

 optional consent

continuous monitoring of interstitial glucose concentrations over 14 days

(x)

(x)

(x)

(x)

patient reported outcomes

 questionnaires completed on site

NAS, GSLTPAQ, PDDS, MSWS-12, ABC-Scale, FSS, FSMC, PROMIS cognitive abilities, BPI, PainDETECT, BDI-II, HAQUAMS, PROMIS general health

x

x

x

(x)

 web-based questionnaires (completed from home)

dietary intake (24-h-recall), FFQ

x

x

x

(x)

quantitative assessment of motor functions

 hand grip force

hand-held dynamometer, 3x each side

x

x

x

(x)

 timed tests

T25-FW, 9-HPT

x

x

x

(x)

 visuo-perceptive motion analysis PASS-MS

short walks, static balance, stand-up-and-sit, stepping in place, finger tapping, finger-nose test

x

x

x

(x)

cognitive assessment

 interview

handedness, education

 Brief International Cognitive Assessment for Multiple Sclerosis (BICAMS)

SDMT, VLMT, BVMT-R

x

x

x

(x)

vision and the visual system

 vision and the visual system

refraction, keratometry, non-contact tonometry, high- and low-contrast visual acuity, perimetry, VEP,

OCT of macula and optic nerve head

x

x

x

(x)

 optional consent

multi-focal VEP, electroretinogram (ERG)

(x)

(x)

(x)

(x)

cerebrospinal magnetic-resonance imaging

 neuroaxis MRI

• cerebral:

MPRAGE, T2-SPACE, FLAIR, MPM, DWI, rsfMRI

• spinal:

STIR (whole spine), PSIR (C2&C3 and C7/T1)

x

x

x

(x)