Ischaemic stroke and Clostridium septicum sepsis and meningitis in a patient with occult colon carcinoma - a case report and review of the literature

Background Clostridium septicum is a rare cause of meningitis and brain abscess in children and adults. Gas production by the pathogen can lead to pneumocephalus and the overall mortality rate of Clostridium septicum CNS infection is as high as 74%. The most common entry site of the pathogen is the gastrointestinal tract. Case presentation We describe a 74-year-old man who presented with a left-sided cerebral infarction in the middle cerebral artery territory. In addition the patient showed signs of Systemic Inflammatory Response Syndrome and Disseminated Intravascular Coagulation. Examination of blood cultures and cerebrospinal fluid led to the diagnosis of sepsis and meningitis caused by Clostridium septicum. Despite appropriate antibiotic therapy the condition of the patient deteriorated rapidly and he died on day 2 after admission. Autopsy revealed a previously unknown adenocarcinoma of the colon ascendens as entry site of the pathogen. Conclusion Clostridium septicum should be considered as potential pathogen in patients with sepsis and meningitis. Gram stain morphology in conjunction with severe sepsis can rapidly point into the direction of this pathogen. CNS infections manifest either as meningoencephalitis/cerebritis or as brain abscess. Entry site of the pathogen is almost uniquely the gastrointestinal tract. In adults more than 50% suffer from colorectal carcinoma, therefore survivors of Clostridium septicum infections should be examined for underlying occult colorectal malignancy.

Here we describe a case of sepsis and meningitis caused by Clostridium septicum complicated with ischemic stroke and review the literature with a focus on pathogen, portal of entry, therapy and outcome.

Case presentation
A 74-year-old man was admitted to our emergency department in a somnolent state of consciousness with left gaze preference, aphasia, and right-sided hemiparesis (National Institutes of Health Stroke Scale, NIHSS-score: 17). The symptoms were first realized after awakening. Cranial computed tomography and angiography revealed a left-sided infarction in the middle cerebral artery (MCA) territory caused by a peripheral MCA occlusion (Fig. 1). In addition the patient showed signs of Systemic Inflammatory Response Syndrome (SIRS) and Disseminated Intravascular Coagulation (DIC) (body temperature 41.1°C, heart rate 140/min, respiratory rate 17/min, leucocytes 4.17 × 10^3/ul, C-reactive protein (CRP) 54.3 mg/l, platelet count 111 × 10^3/ul). Intravenous fluid therapy was started immediately. After acquisition of blood cultures and cerebrospinal fluid (CSF), an empiric antibiotic therapy with ceftriaxone, ampicillin and aciclovir was initiated. Soon after, the patient required cardiopulmonary resuscitation in the setting of septic shock.
After intubation and cardiopulmonary stabilization the patient was admitted to the neuro-intensive care unit. CSF examination showed 390 leucocytes/ml, elevated lactate (5.52 mmol/l) and protein levels (2783 mg/l) as well as normal glucose levels (66% of serum levels). Immediate Gram staining of CSF revealed partially elongated, Grampositive rods without spore formation. Because of their size and appearance Listeria spp. could be ruled out and Bacillus spp. or Clostridium spp. were suspected (Fig. 2a). Therefore the antibiotic therapy was escalated with vancomycin. On the following day anaerobic blood cultures grew also Gram-positive rods with some of them showing endospore formation (Fig. 2b). Aerobic blood cultures remained sterile. Accordingly, sepsis and meningitis by Clostridium spp. were assumed. Culture results confirmed this tentative diagnosis and the bacteria were identified by mass spectroscopy as Clostridium septicum. Antibiotic susceptibility testing showed that the strain was sensitive to penicillin G, imipenem, clindamycin, vancomycin and metronidazole with minimal inhibitory concentration of 0.023 mg/l, 0.006 mg/l, 0.032 mg/l, 0.75 mg/l and 0.25 mg/ l, respectively. Respiratory specimens and urine were unremarkable. Transthoracic echocardiography did not show endocarditis and there was no sign of an infection focus in ear, nose, throat or the sinuses. The further course was characterized by a persistent severe septic disease pattern with high demand of catecholamines, strong increase of inflammation parameters (leucocytes max. 42.86 × 10 3 /ul, procalcitonin (PCT) max. 177.32 ng/ml, CRP max. 398.8 mg/l). The antibiotic therapy was switched to high dose penicillin G and clindamycin. The inflammatory parameters started to decrease approximately 48 h after initiation of empiric antibiotic therapy. However, because the patient showed a persistent loss of brain stem reflexes after initial cardiopulmonary resuscitation, it was decided to change the therapy to a palliative regimen. The patient died two days post admission. Due to the fact that no obvious risk factors for Clostridium septicum bacteremia were present (e.g. infected wounds, myonecrosis, diabetes mellitus, severe atherosclerotic cardiovascular disease, neutropenia, liver cirrhosis), a previously unknown gastrointestinal focus of infection was most likely [4]. Accordingly, autopsy revealed an adenocarcinoma of the colon ascendens with 2.8 cm in diameter as entry site of the pathogen.

Discussion
The leading neurological symptoms of our patient were those of a left sided stroke in the MCA-territory which was confirmed by cranial computed tomography and angiography.
At the same time the patient suffered from bacterial sepsis and meningitis. The question in dispute is what came first? One order of events is that Clostridium septicum crossed the gastrointestinal barrier and entered the blood stream with the colon carcinoma as entry site. From the blood it spread to the CNS causing meningitis. In parallel, the sepsis lead to a DIC which on its part resulted in hypercoagulation and cerebral infarction. The stroke would have been a consequence of invasive clostridial infection.
A second possibility is that the stroke was the initial event leading to a compromised immune response and/or by stress-mediated paralytic ileus to an impaired intestinal barrier [5]. This enabled Clostridium septicum to enter the blood stream and supported by the stroke-induced damage of the blood-brain barrier (locus minoris resistentiae) to cause CNS infection. The invasive clostridial infection would have been a consequence of the stroke.
Two facts argue against the second hypothesis. First, the stroke was a wake-up stroke and sepsis and DIC were already apparent at admission. Therefore, the stroke induced impairment of the intestinal barrier must have developed within a couple of hours which is rather unlikely. Second, no bacteria were detected microscopically in the stroke area during brain autopsy and the published cases of Clostridium septicum CNS infections showed that the pathogen does not need an impaired blood-brain barrier to cause meningitis.
In our opinion the first hypothesis is more likely although we cannot rule out the second sequence of events with certainty.
The first hint on the nature of the pathogen was its CSF Gram stain morphology. All the major pathogens can be distinguished by their different appearance in the Gram staining. Only Listeria monocytogenes is Gram-positive and rod-shaped. However, size and shape of the Gram-positive rods in our patient pointed towards Bacillus or Clostridium spp. as pathogens. To cover possible Bacillus-infection the Fig. 2 Gram stain morphology of Clostridium septicum from CSF (a) and blood culture (b) in 1000× magnification. Interestingly, spore formation (arrow) was only observed in Gram stains from blood cultures addition of vancomycin to the empiric antibiotic regimen with ampicillin and ceftriaxon was reasonable.
Clostridia are obligately anaerobic, gram-positive rods. Because of their ability to produce environmentally resistant endospores they are widespread in nature and are commonly found in soil and the intestinal tracts of humans and animals. Clostridia produce more kinds of protein toxins than any other bacterial genus causing diseases like tetanus, botulism, enterocolitis and anaerobic myonecrosis (gas gangrene). One important species is Clostridium septicum. Due to production of its 4 major exotoxins (α-, β-, γand δ-toxin) it is able to cause myonecrosis. In line with Clostridium perfringens and Clostridium tertium it is the third most common Clostridium spp. causing sepsis [4]. Patients with bacteremia by this organism are usually severely ill and mortality rates rise up to 70% [3]. More than 50% suffer from some kind of gastrointestinal pathology, such as diverticular disease, or an underlying malignancy, such as colorectal carcinoma. Further risk factors for clostridial bacteremia include neutropenia with or without enterocolitis, diabetes mellitus or gas gangrene [4]. Whether Clostridium septicum colonizes the human gastrointestinal tract is still under debate. However, a breach in the mucosal barrier for example by enterocolitis or colorectal cancers is required for invasive infection [3].
A rare complication of Clostridium septicum bacteremia is a hematogenous spread to the CNS. CNS infections manifest either as meningoencephalitis/cerebritis or as brain abscess [2]. Because of the ability of Clostridium septicum to produce gas a pneumocephalus may result that can be localized or diffuse [6][7][8]. Brain autopsy in our patient confirmed a haemorrhagic infarction in the left MCA territory. There were signs of diffuse inflammation at supra-and infratentorial areas in terms of a meningoencephalitis. An infectious agent and/or gas production could not be verified at microscopic level. According to our literature review the overall mortality in patients with Clostridium septicum CNS infection is 74% (5 survivors, 14 deceased) and most patients died within the first 48 h (79%). Interestingly, the mortality of brain abscess with the possibility of a surgical intervention is significantly lower than that of meningoencephalitis with antibiotic therapy alone (17% versus 100%, p = 0.001).
Therefore, therapeutic management of Clostridium septicum CNS infection consists of antibiotic therapy and, if possible, surgery/aspiration of the brain abscess. Antibiotics with low MICs are penicillin G, cefazolin, clindamycin and rifampin. A combination therapy of penicillin plus clindamycin or rifampin might be beneficial [9]. Prognosis of meningoencephalitis with antibiotic therapy alone is poor and it seems essential for survival that a localized process (e.g. an abscess) exists that is accessible to surgical interventions.

Conclusions
Clostridium septicum should be considered as potential pathogen in patients with sepsis and meningitis. Gram stain morphology in conjunction with severe sepsis can rapidly point into the direction of this pathogen. Detailed microbiological analysis is required for identification and appropriate antibiotic therapy. If a localized process in the CNS is present a surgical intervention seems to be essential for survival. Adult patients with Clostridium septicum infection should be examined for underlying occult gastrointestinal disease or cancer.